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KMID : 0380019950100050533
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 5 p.533 ~ p.538
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Recovery of Poly(3-hydroxybutyrate) from Recombinant Escherichia coli by Autolysis with Bacteriophage Lambda
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정옥희/Jeung, Ok Hee
한세광/장용근/이상엽/Hahn, Sei Kwang/Chang, Yong Keun/Lee, Sang Yup
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Abstract
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An autolytic system based on a thermally inducible phage lambda, AHL1, has been applied for the recovery of poly(3-hydroxybutyrate) [PHB] from a recombinant Escherichia colt, XL1-Blue, harbouring a plasmid (pSYL105) containing the Alcaligenes eutrophus PHB biosynthesis genes. The lytic capability of Al-IL1 was evaluated in flask culture for both lysogens, XL1-Blue (AHL1) and XL1-Blue (AHL1, pSYL105). When the optical density of culutre at 600nm(ODsoo) reached 0.;cell lysis was induced by increasing the temperature from 301C to 42°C. Most cells of XL1-Blue (AHL1) were lysed by the autolytic system in an hour after the thermal induction, while the lytic efficiency was slightly lower for XL1 -Blue (AHL1, pSYL105). The existence of pSYL105 in cells seemed to inhibit, to some extent, the lytic capability of AHL1 even at low PHB content. The lytic efficiency remarkably decreased as the induction was delayed to allow PHB accumulation. When a chemical induction using 2% (v/v) chloroform was introduced after an hour of thermal induction, we could obtain a good lytic efficiency.
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KEYWORD
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